Abstract
Efficient aminoacylation of an RNA minihelix (a proposed precursor of tRNA) was achieved without any ribozymes or protein enzymes. The reaction was promoted with a template-like bridging Oligonucleotide and a 5'-aminoacyl-phosphate-oligonucleotide. Aminoacylation was template-dependent and showed high preference for a particular ribose hydroxyl. The 5'-aminoacyl-phosphate-oligonucleotides have the same adenylate linkage as in aminoacyl-AMP--the universal intermediate used in modern aminoacylation systems with aminoacyl-tRNA synthetases. Thus, the results raise the possibility that this kind of system could have played a role in the transition from the RNA world to the theatre of proteins.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
