Abstract
It has been found that mnay hydrophobic substances such as hydrocarbons and glyceride oils may not completely elute from a reversed-phase liquid chromatographic column when using semi-aqueous mobile phases containing methanol or acetonitrile. Addition of lower-polarity solvents such as methylene chloride or tetrahydrofuran to the carrier causes rapid elution of these non-polar susbtances. By using a highly retentive reserved-phase packing. Zorbax ODS, it has been demostrated that non-aqueous mobile phases provide an effective way of separating water-insoluble, non-polar substances. The enhanced solubility of compounds in these non-aqueous solvents greatly facilitates the detection of compounds which have poor UV absorbance/fluorescence characteristics.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
