Abstract
Discussion and SummaryA series of precipitin reactions in agarose gel are described which resemble, but are unrelated to antigen-antibody reactions commonly studied by similar methods. This appears to be due to the lack of interference in agarose by sulfate groups present in ordinary agar. The reactions appear to be due to complexes formed between highly negatively charged compounds such as dextran sulfate, heparin, and inositol phosphatide and the highly positively charged polymyxin and aminoglycoside antibiotics, lysozyme, protamine, DEAE-dextran, and presumably beta lipoproteins as well. Unfortunately, the formation of a precipitin band between human serum and dextran sulfate and sensitivity limited to antibiotic concentrations several-fold higher than achieved during antimicrobial therapy, prevents direct adaptation of these methods to a rapid procedure for detecting basic antibiotics in serum. The approach could conceivably be adapted to measurement of basic drugs in urine or to ultrafiltrates of ser...
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Schedule a callMore From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
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