Non-antibiotics matter: Evidence from a one-year investigation of livestock wastewater from six farms in East China

Abstract

Livestock wastewater is an important source of pharmaceuticals in aquatic environments; however, most related studies only focused on antibiotics. This study investigated 18 pharmaceutical active compounds (PhACs), including 12 antibiotics and 6 non-antibiotics, in livestock wastewater during a one-year survey of six livestock farms in East China. The results showed that four non-antibiotic PhACs—caffeine, N,N-diethyl-m-toluamide, gemfibrozil, and diclofenac—exhibited high detection frequencies (80% to 97%), high concentrations (median 0.43 to 3.79 μg/L), poor removal efficiencies (3% to 53%), and high environmental risks. A ranking system was developed to prioritize PhACs based on their occurrence, removal, and environmental risks in livestock wastewater; diclofenac, N,N-diethyl-m-toluamide, sulfamethazine, sulfadiazine, and gemfibrozil, were identified as the top five priority PhACs that should be considered first. Finally, a preliminary source apportionment protocol using four priority PhACs was proposed to trace the emission originating from treated and untreated livestock wastewater and to indicate the major contributor (cattle or swine farms) in the region. To the best of our knowledge, this is the first long-term investigation on the pollution characteristics of non-antibiotics in livestock wastewater in China, and our findings highlight the importance of considering non-antibiotics and the prioritized PhACs for the pollution control of PhACs in livestock wastewater.