Non-animal Peptone for Serum Free Cultivation of Recombinant Mammalian and Animal Cells

Abstract

Gene expression by large-scale transfection of mammalian and animal cells is becoming an establishedtechnology for the fast production of therapeutic biotech products. However, efforts are still needed to optimizeproduction parameters in order to maximize volumetric productivity while maintaining product quality.Generally, in cell culture 5% to 15% serum is used as supplement in the standard basal media in addition to saltsand amino acids for healthy cell growth. To overcome the serum related problems, serum derived and animalderived substances are being used. The serum, serum derived or animal derived substances have someadvantages and disadvantages which we have summarized in this review. This article mainly focuses on thereplacement of serum, serum derived or animal derived substance with plant derived peptones to fulfill theregulatory requirements.In addition, this article also highlights the methods of peptone selection, peptone screening and some issuesrelated to peptone utilization and their solutions.On the basis of review, we found that the usage of ultrafiltered plant peptone in combination with polyaminecould be the best composition for cells growth and reproducible expression of the therapeutic Bio- products. Thesource or type of peptone (soy peptone, wheat and rice peptone) and polyamines (cadaverine, putrescine,spermidine, spermine, agmatine and ornithine etc) are cell dependent and can be selected on the basis of theirperformance with respect to cell growth, expression of protein of interest with consistency in the productionprocess.