Abstract
Dissolved non-polar gases (five noble, three diatomic, and four unbranched aliphatic hydrocarbon gases) were resolved by aqueous high-performance liquid chromatography using an ion-moderated partition chromatography column (Bio-Rad HPX-87P) originally designed for monosaccharide analysis. The dissolved-gas peaks, which were detected and quantitated by high-sensitivity refractive-index detection, are eluted at 25°C in the order Ne, He, N 2, H 2, O 2, Ar, CH 4, Kr, Xe, C 2H 6, C 3H 8, n-C 4H 10. Based on the general elution order, and on the temperature dependences (4–72°C) of elution times for the individual gases, a mixed chromatographic mechanism is proposed, in which separation of the gases results from a mixture of size-exclusion, ion-ion-induced dipole, and classical hydrophobic effects, plus a second type of “hydrophobic” effect involving binding of the clathrate hydrate cages surrounding non-polar solutes to similar cages formed around hydrocarbon portions of the column packing material.
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