Abstract
The aim is to study changes in the activity of distinct NO-synthase isoforms of blood lymphocytes in persons with acne vulgaris, for those individuals with disease accompanied by seeding of film-forming and planktonic staphylococcus forms. Enzyme activity was determined both before and after treatment. Methods. 44 patients with acne vulgaris were studied, for that persons the cultures of filmforming and planktonic forms of Staphylococcus aureus and Staphylococcus epidermidis were isolated from purulent pustules. Separation of lymphocytes from fresh peripheral heparinized blood of patients (before and after treatment) and individuals in the control group was done in the ficol triumbras (ρ=1.08 g/cm3 ). NO-synthase activity was expressed as citrulline production in picomoles per 1 milligram of protein per 1 minute. Results. Slightly higher rates of leukocyte іNOS were detected with the participation of biofilm staphylococci for S. aureus and S. epidermidis species compared to the planktonic forms of these staphylococcal species. For the acne caused both by S. aureus and S. epidermidis (planktonic and film-forming form) it was shown the significant increase ofendothelial NO-synthase activity (3.8–5.2 times) and inducible NO-synthas activity (52.5–55.1 times) ofperiferial blood leukocytes compared to control. The activity of the inducible isoform NO synthase significantly decreases relative to the control level after the course of acne treatment complicated by the proliferation of S. aureus (planktonic and film-forming form). Conclusions. The increase in the activity level of iNOS was observed with the participation of biofilm staphylococci of S. aureus and S. epidermidis species. In both forms of acne caused by S. aureus and S. epidermidis (planktonic and film-forming), the activity of NO-synthase isoforms increases with respect to control values. The activity of the inducible isoform NO-synthase can serve as a biomarker for monitoring treatment efficacy.
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