Abstract
The presence of a soluble form of the common cytokine receptor γ chain (γ c) in cell free supernatants from unstimulated and 1–6 days PHA stimulated peripheral blood lymphocyte (PBL) cultures was analyzed using a sandwich ELISA. No naturally produced soluble γ c could be detected in these cell free culture supernatants, although a sensitivity in the nanogram range was achieved for recombinant baculovirus expressed human soluble γ c with this assay (detection limit 0.2 ng hIL-2 sRγ). Analysis of the very same supernatants for soluble IL-2Rα demonstrated increased concentrations (up to 20.4 ng/ml) of this other IL-2R member. The membrane-associated form of the common cytokine receptor γ chain was detected in cell lysates prepared from stimulated PBL at a concentration of 3.5 ng per 0.5×10 6 cells. Analysis of a small panel of serum samples from patients with different disorders verified that the soluble form of hIL-2Rα, but not hIL-2 sRγ, can be detected, which thereby strongly suggests that the human soluble γ c seems to be a valuable marker only for a limited number of clinical disorders.
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