No positive cross-resistance to Cry1 and Cry2 proteins favors pyramiding strategy for management of Vip3Aa resistance in Spodoptera frugiperda.

Abstract

Pyramided Bacillus thuringiensis (Bt) crops could delay insect resistance development by expressing multiple distinct Bt proteins to manage the same insect pest. The efficacy of pyramiding strategy for resistance management could be jeopardized by positive cross-resistance, which is defined as insects showing resistance to one Bt protein also exhibiting resistance to other Bt proteins. The fall armyworm, Spodoptera frugiperda, is a destructive agricultural pest and target of Vip3Aa. In this study, we evaluate the cross-resistance of Vip3Aa resistance in S. frugiperda to Bt cotton and corn plants, as well as purified Bt proteins. Diet bioassay showed that Vip3Aa-resistant (RR), -heterozygous (RS), and -susceptible (SS) insects of S. frugiperda performed similarly against Cry2Ab2 purified protein. The data also indicated that genotypes RR and RS were more susceptible to Cry1F and Cry2Ae purified proteins relative to SS. The diet bioassays suggested that resistance to Vip3Aa does not confer any positive cross-resistance to Cry1F, Cry2Ae or Cry2Ab2 in S. frugiperda. The plant bioassay indicated that the S. frugiperda resistance to Vip3Aa conferred no cross-resistance to corn and no cross-crop resistance to cotton plants expressing Cry1 and/or Cry2 proteins. These findings indicate that a lack of positive cross-resistance to Cry1 and Cry2 proteins favors pyramiding strategy for managing S. frugiperda resistance to Vip3Aa protein. © 2020 Society of Chemical Industry.