Abstract
Background Platelets are specialized anucleate cells that play key roles in hemostasis through their ability to rapidly adhere to subendothelial matrix proteins and endothelial cells (platelet adhesion) and to other activated platelets (platelet aggregation). Platelet activation is an irreversible process resulting ultimately in platelet apoptosis and death. The NO-cGMP-PKG pathway plays an essential role in platelet inhibition, however the role of this pathway in regulation of platelet apoptosis has not been investigated in detail so far. Surface exposure of negatively charged phospholipid phosphatidylserine (Annexin V-binding) and loss of mitochondrial membrane potential (ΔΨm) of activated platelets were used as markers of platelet apoptosis induced by thrombin (up to 0.5 U/ml), convulxin (Cvx 5 ng/ml) or by a mixture of thrombin/convulxin (Thr/Cvx 0.005 U/ml/5 ng/ml).
Highlights
Platelets are specialized anucleate cells that play key roles in hemostasis through their ability to rapidly adhere to subendothelial matrix proteins and endothelial cells and to other activated platelets
The NO-cGMP-PKG pathway plays an essential role in platelet inhibition, the role of this pathway in regulation of platelet apoptosis has not been investigated in detail so far
Surface exposure of negatively charged phospholipid phosphatidylserine (Annexin V-binding) and loss of mitochondrial membrane potential (ΔΨm) of activated platelets were used as markers of platelet apoptosis induced by thrombin, convulxin (Cvx 5 ng/ml) or by a mixture of thrombin/convulxin (Thr/Cvx 0.005 U/ml/5 ng/ml)
Summary
Platelets are specialized anucleate cells that play key roles in hemostasis through their ability to rapidly adhere to subendothelial matrix proteins and endothelial cells (platelet adhesion) and to other activated platelets (platelet aggregation). NO inhibits platelet apoptosis by cGMP-dependent and-independent pathways Natalia Rukoyatkina, Ulrich Walter and Stepan Gambaryan* Open Access from 4th International Conference of cGMP Generators, Effectors and Therapeutic Implications Regensburg, Germany.
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