No increase in free fetal DNA level in ectopic pregnancy: A preliminary study

Özge Kömürcü Karuserci,Seyhun Sucu,Özcan Balat,Mete Gürol Uğur

doi:10.4274/tjod.54715

Özge Kömürcü Karuserci, Seyhun Sucu + Show 2 more

Open Access

https://doi.org/10.4274/tjod.54715

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Abstract

Objective:The aim of this study was to diagnose ectopic pregnancy in the early period by measuring cell-free fetal DNA (cffDNA) levels in maternal blood using spectrophotometry.Materials and Methods:Thirty patients with ectopic pregnancy and 30 patients with first trimester intrauterine pregnancy were enrolled in this prospective controlled study. cffDNA levels in maternal serum were measured using spectrophotometry.Results:There were no differences between the two groups in terms of cffDNA absorbance levels.Conclusion:Spectrophotometry is not suitable for measuring cffDNA levels to diagnose ectopic pregnancies in the early period. Practical and cost-effective methods should be found or larger patient series should be investigated.

Highlights

Ectopic pregnancy is complication of pregnancy with high morbidity and mortality rates, which is why early and precise diagnosis is very important
*: Abnormal values in both groups absorbance using spectrophotometry, but we could found differenced between intrauterine the pregnancy and ectopic pregnancy groups in terms of cell-free fetal DNA (cffDNA) absorbance
We found that the cffDNA values did not increase with increasing hCG values

Summary

Introduction

Ectopic pregnancy is complication of pregnancy with high morbidity and mortality rates, which is why early and precise diagnosis is very important. The current administration for diagnosis of ectopic pregnancy includes serial serum beta human chorionic gonadotropin (β-hCG) levels and transvaginal ultrasound[1]. Several checks for β-hCG level and ultrasound monitoring are required for diagnosis and management decisions. Another marker could be used in diagnosis and management. Cell-free nucleic acids have been studied for use as potential candidate biomarkers for numerous conditions, especially in gynecologic cancers, ovarian and endometrial diseases, obstetric disorders such as preeclampsia[3], fetal aneuploidy, intrauterine fetal demise, and abortus[4]. Concentration of cell-free nucleic acids in serum of patients undergoing in vitro fertilization

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