Abstract
Studies on heterokaryons forced between sorbose-resistant (transport-defective) mutans of Neurospora crassa and a sorbose-sensitive (transport-intact) wildtype strain show that mutants sorr A-1 and sorr B-57, mapping in separate linkage groups, are recessive to wildtype; a third mutant, not linked to the others, shows intermediate expression of the resistance character for one of two criteria considered. The functional relations between sorr-loci A and B and the amount of resistance caused by defects in both of them have been investigated in forced heterokaryons of the type A-B + A-B, AB-+AB- and A-B + AB-. Pairwise combination of A- with B- resulted in complementation to the sorbose-sensitive wild-phenotype (“no-growth” complementation). The occurrence of no-growth complementation in the double heterozygote supports the conclusion derived earlier that both gene loci A and B are coding for permeases responsible for the uptake of sorbose.
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