No effect of plant growth retarding compounds and growth stimulators on indolo-3-acetic acid oxidase activity in greening cucumber cotyledons

Abstract

Cotyledons dissected from 5-day-old etiolated cucumber seedlings were incubated in solutions on AMO-1618, B-Nine, CCC and Phosfon D for 48 h in light. In some tests the retardants were applied in mixed solutions with GA<sub>3</sub> or BAP. IAA oxidase was extracted and purified by means of molecular sieving through a bed of Sephadex G-25. The retardants inhibited chlorophyll synthesis by 50 % or more, and had essentially no effect on IAA oxidase activity per cotyledon basis. GA<sub>3</sub> and BAP also had no effect on enzyme activity in spite of a fact that the compounds stimulated growth of the cotyledons. The crude enzyme extract from B-Nine treated cotyledons showed lower IAA oxidase activity in comparison with the water treated control, the effect being due to a longer lag-phase preceding the initiation of IAA oxidation. KNO<sub>3</sub> strikingly stimulated expansional growth of the cotyledons, the effect being correlated with the accelerated chlorophyll accumulation. KNO<sub>3</sub> had no effect on IAA oxidase activity per cotyledon and decreased it per gram fr wt. It is concluded that [1] the growth rate of cucumber cotyledons is not correlated with IAA oxidase activity, and ;[2] the growth retarding compounds do not affect IAA oxidase system is this tissue.