Abstract
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H, 31P-NMR spectroscopy, FPLC and gel electrophoresis. An enexpected finding was the high level of 2′,3′-cyclic nucleotides, detected by characteristic 31P-NMR resonances in the region 20–21 ppm, originating from the yeast component. 31P-NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.
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