NMR Structural Studies of the Supramolecular Adducts between a Liver Cytosolic Bile Acid Binding Protein and Gadolinium(III)-Chelates Bearing Bile Acids Residues: Molecular Determinants of the Binding of a Hepatospecific Magnetic Resonance Imaging Contrast Agent

Abstract

The binding affinities of a selected series of Gd(III) chelates bearing bile acid residues, potential hepatospecific MRI contrast agents, to a liver cytosolic bile acid transporter, have been determined through relaxivity measurements. The Ln(III) complexes of compound 1 were selected for further NMR structural analysis aimed at assessing the molecular determinants of binding. A number of NMR experiments have been carried out on the bile acid-like adduct, using both diamagnetic Y(III) and paramagnetic Gd(III) complexes, bound to a liver bile acid binding protein. The identified protein "hot spots" defined a single binding site located at the protein portal region. The presented findings will serve in a medicinal chemistry approach for the design of hepatocytes-selective gadolinium chelates for liver malignancies detection.