NMR spectroscopy for assessing lipid oxidation

Abstract

Although lipid oxidation involves a variety of chemical reactions to produce numerous substances, most traditional methods assessing lipid oxidation measure only one kind of oxidation product. For this reason, in general, one indicator of oxidation is insufficient to accurately describe the oxidation status of lipids, and two or more methods should be used for the accurate assessment of lipid oxidation. An 1H NMR method that can quantitatively track the disappearance of triacylglycerol starting materials during the oxidation process has been found to very a reliable method, which gives a satisfactory description of lipid oxidation status throughout the entire course of oxidation, regardless of the kinds of oxidation products produced. It was found that the NMR method not only correlates well with traditional methods, but also it offers additional advantages such as higher accuracy, non‐destructiveness, and a faster and more convenient procedure.