Abstract
Kiwifruits have become one of the most common food sources triggering allergic reactions. In patients suffering from birch pollen related food allergy, reactions result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1, followed by immunological cross-reactivity to structurally homologous proteins in kiwifruit. Clinical symptoms range from scratching and itching of the oral cavity to more severe immunological reactions such as rhino conjunctivitis. In this work we assigned backbone and side chain 1H, 13C and 15N chemical shifts of the 17 kDa PR-10 allergens Act c 8.0101 and Act d 8.0101 from golden (Actinidia chinesis) and green (Actinidia deliciosa) kiwifruit by solution NMR spectroscopy. The chemical shift data confirm the characteristic Bet v 1 fold for both proteins, consisting of a seven-stranded antiparallel β-sheet interrupted by two short α-helices, along with a long C-terminal α-helix. Our data provide the basis for determining the three-dimensional solution structures of these proteins and characterizing their immunological cross-reactivity on a structural basis.
Highlights
The most common allergy in Western and Central Europe is pollinosis, elicited by sensitization to birch pollen
Patients suffering from birch pollinosis frequently experience allergic reactions after consumption of certain fruits and nuts, due to immunological cross-reactivity of Bet v 1 specific antibodies (IgE) to proteins that are present in these food sources
Immunological cross-reactivity to birch pollen is associated with the class 10 pathogenesis related (PR) proteins, Act c 8 and Act d 8, from golden (Actinidia chinensis) and green (Actinidia deliciosa) kiwifruit (Oberhuber, 2008)
Summary
The most common allergy in Western and Central Europe is pollinosis, elicited by sensitization to birch pollen. Patients suffering from birch pollinosis frequently experience allergic reactions after consumption of certain fruits and nuts, due to immunological cross-reactivity of Bet v 1 specific antibodies (IgE) to proteins that are present in these food sources. Immunological cross-reactivity to birch pollen is associated with the class 10 pathogenesis related (PR) proteins, Act c 8 and Act d 8, from golden (Actinidia chinensis) and green (Actinidia deliciosa) kiwifruit (Oberhuber, 2008). The expression of these proteins, which are believed to play a major role in plant defense, can be induced by pathogenic or environmental stress. In this work we present the solution NMR backbone and side-chain assignments of the two recombinantly expressed allergens Act d 8 and Act c 8
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