Abstract
The Linderstrøm-Lang model describes proton exchange as proceeding from an ‘open’ state which is in equilibrium with a ‘closed’ state from which exchange cannot occur. This predicts a biexponential exchange process, although limiting conditions can give rise to monoexponential exchange profiles. When monoexponential exchange profiles are observed, insufficient information is present to define the exchange mechanism. We have directly observed a biexponential exchange process, enabling us to distinguish between alternative amide proton exchange models. Our results are not compatible with the standard local unfolding model, in which units of secondary structure open in a concerted fashion, with each of the newly exposed amide protons exchanging at the same rate which they would in random coil peptides.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
