Abstract
METHODS: Two human pancreatic cancer cell lines (AsPC-1 and S2-013) were treated with varying concentrations (0.1-100mcM) of peptide. Proliferation was measured by 3H-thymidine incorporation and cell counting at different time points (24-72h). Cell cycle analysis was determined by flow cytometry with propidium iodide DNA staining. Cell cycle-associated proteins were investigated by western blotting. Apoptosis was confirmed by annexin-binding assays.
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