NMR‐Based Metabolomic Profiles of Mice Fed a High Fat Diet and Supplemented with Resistant Starch and/or Lactobacillus

Abstract

Incidence of obesity and type 2 diabetes mellitus (T2DM) is steadily increasing, and the contributions of diet and the gastrointestinal microbiota to these diseases are only partially understood. Enriching particular populations of microbes in a mouse model of pre‐diabetes and monitoring systemic metabolic effects will help to elucidate the mechanisms of obesity and T2DM progression. C57BL/6J mice were fed a 45% high fat diet for nine weeks and then supplemented with type 2 resistant starch (RS), Lactobacillus plantarum (Lp), or both for six weeks (n=10 per group). Urine was collected throughout the experiment; cecal contents and serum were obtained at the time of mouse sacrifice. All samples were analyzed using NMR‐based metabolomics. Unsupervised principal component analysis showed clear separation between RS and non‐RS groups for both cecal and serum samples. Significant differences were observed for microbial metabolites and amino acids when comparing RS groups to control, as well as when comparing Lp supplementation alone to RS supplementation alone. These results have implications for monitoring the effects of dietary supplementation on the gastrointestinal microbiota and overall host health. Funding was provided by the American Diabetes Association.