N-methyl-d,l-aspartate stimulates growth hormone and prolactin but inhibits luteinizing hormone secretion in the pig

Abstract

The effects of n-methyl-d,l-aspartate (NMA), a neuroexcitatory amino acid agonist, on luteinizing hormone (LH), prolactin (PRL) and growth hormone (GH) secretion in gilts treated with ovarian steroids was studied. Mature gilts which had displayed one or more estrous cycles of 18 to 22 d were ovariectomized and assigned to one of three treatments administered i.m.: corn oil vehicle (V; n=6); 10 μg estradiol-17b/kg BW given 33 hr before NMA (E; n=6); .85 mg progesterone/kg BW given twice daily for 6 d prior to NMA (P 4; n=6). Blood was collected via jugular cannulae every 15 min for 6 hr. Pigs received 10 mg NMA/kg BW i.v. 2 hr after blood collection began and a combined synthetic [Ala 15]-h GH releasing factor (1–29)-NH 2 (GRF; 1 μg/kg BW) and gonadotropin releasing hormone (GnRH; .2 μg/kg BW) challenge given i.v. 3 hr after NMA. NMA did not alter LH secretion in E gilts. However, NMA decreased (P<.02) serum LH concentrations in V and P 4 gilts. Serum LH concentrations increased (P<.01) after GnRH in all gilts. NMA did not alter PRL secretion in P 4 pigs, but increased (P<.01) serum PRL concentrations in V and E animals. Treatment with NMA increased (P<.01) GH secretion in all animals while the GRF challenge increased (P<.01) serum GH concentrations in all animals except in V treated pigs. NMA increased (P<.05) cortisol secretion in all treatment groups. These results indicate that NMA inhibits LH secretion and is a secretagogue of PRL, GH and cortisol secretion with ovarian steroids modulating the LH and PRL response to NMA.