Abstract
Amperometric studies have indicated that substance P as well as NMDA stimulates release of NO in rat aortic rings. These data have been confirmed by functional observations of vaso-relaxant action of NMDA within noradrenaline pre-contracted aortic rings, supporting the presence of NMDA receptor in rat aortic rings. It is known that the enzyme endothelial NO synthase (eNOS) mediates vasodilatation not only in rats, but also in C57BL6 mice aortic ring, indicating that in this blood vessel NO is the endogenous endothelium-derived vasodilator. In this work, amperometry together with specifically nitrites insensitive micro-biosensors have been applied to examine the effect of NMDA and substance P upon NO release in rat and in two strains of mice aortic rings. The electrochemical data monitored demonstrate that NMDA mediates vascular relaxation via NO in rats but not in mice. These results are supported by functional data, therefore they suggest that NMDA receptors are “not responding” within these experimental conditions in mice aortic rings.
Highlights
In vivo studies have demonstrated the possibility to monitor NOx [mixture of nitrites and nitrates, i.e. metabolites of nitric oxide (NO)] by means of in vivo microdialysis in rat brain [1]
These results provided direct evidence for NOx release in response to NMDA receptor activation in the cerebellar cortex of adult rat [1]
It appeared that basal amperometric NO related current levels can be monitored in rats as well as in mice aortic rings
Summary
In vivo studies have demonstrated the possibility to monitor NOx [mixture of nitrites and nitrates, i.e. metabolites of nitric oxide (NO)] by means of in vivo microdialysis in rat brain [1]. Amperometric studies have indicated that substance P as well as NMDA stimulates release of NO in rat aortic rings. Amperometric experiments performed together with selective NO detecting mCFE in rat aortic rings, did provide evidence that oxidation of NO can be directly and selectively measured within the lumen of such peripheral tissue [3,4].
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