Abstract
N- Methyl- d-aspartate (NMDA)-induced translocation of protein kinase C (PKC) from cytosol to membrane fractions was examined by the methods of [ 3H]phorbol 12,13-dibutyrate binding and western blotting in rat hippocampal slices. NMDA and l-glutamate induced translocation of PKC from cytosol to membrane fractions in immature rat hippocampal slices, but not in mature ones. The NMDA-induced translocation of PKC was dependent on Ca 2+. It was inhibited by the NMDA receptor antagonists, 2-amino-5-phosphonovaleric acid and ketamine, but not by Mg 2+ and Zn 2+. These results suggest that stimulation of NMDA receptors enhances Ca 2+ influx and thereby induces translocation of PKC in immature rat hippocampus.
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