Abstract
Simple SummaryIn this study, we aimed to identify the putative host cell receptor for Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) CP(Capsid Protein) in the gill membrane of LitoPenaeus vannamei. We established that NM23 is a host cell binding partner for IHHNV CP. Our study is probably the first to address the host cell IHHNV receptor and could provide novel insights into the pathogenesis of IHHNV. We feel that this paper is of interest to the readers of Animals.The aim of this study was to identify the putative host cell receptor for Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) CP in the gill membrane of L. vannamei. Putative CP binding partners were screened first using a 2-dimensional Virus Overlay Protein Blot Assay (VOPBA) to probe isolated gill membrane proteins using recombinant CP. Putative binding partners were identified using mass spectrometry. A Phage Display Random Dodecapeptide Library was used to screen for dodecapeptides and motifs that bound to CP. Finally, putative binding pairs were confirmed using GST(glutathione-S-transferase) pulldown assays. 2-Dimensional VOPBA identified NM23 as a putative binding partner for IHHNV CP. GST pulldown experiments confirmed the direct interaction of NM23 and IHHNV CP. The phage display library was used to identify six groups of dodecapeptides that bound to CP. From these peptides, three characteristic binding motifs were identified, SW*Y, SKWV, and PQR. Interestingly, the SW*Y motif was also found in NM23. We are the first to implicate NM23 in IHHNV infection and postulate that it may bind to the CP using the SW*Y motif, although this remains to be confirmed.
Highlights
Infectious Hypodermal and Hematopoietic Necrosis (IHHN), known as Runt-Deformity Syndrome (RDS), is caused by the IHHN virus (IHHNV) and was identified in breeding shrimp (LitoPenaeus stylirostris) in Hawaii, USA in 1981 [1]
The relevant information about Nucleoside Diphosphate Kinase (NDPK) from MS + MS/MS was shown as Table 1
The nucleoside diphosphate kinase group I (NDPK-I)-like domain is found between 64–453 bp
Summary
Deformity Syndrome (RDS), is caused by the IHHN virus (IHHNV) and was identified in breeding shrimp (LitoPenaeus stylirostris) in Hawaii, USA in 1981 [1]. The virus is one of the most serious shrimp diseases and has a wide host range. The virus infects ectodermal organs, such as the gills epidermis, intestinal epithelial cells, nerve cord, and ganglion, and mesodermal organs, such as the hematopoietic tissue, antennal gland, gonads, lymphoid organs, connective tissue, and striated muscle [2,3]. IHHNV is a non-enveloped, linear, single-stranded DNA virus belonging to Brevidensovirus Densovirinae Parvoviridae. IHHNV is the smallest known shrimp virus, reaching only 22 nm in diameter and containing 4.1 kb of DNA [4]. The virus contains three open reading frames (ORFs) and non-coding sequences in two terminals. The World Organization for Animal Health (OIE) listed IHHNV as a key crustacean virus that must be declared
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
