Abstract
Abstract Recurrent hydatidiform molar pregnancies are characterized by uncontrolled proliferation of trophoblasts resulting in the failure of the embryo and placenta to develop properly. Previous studies genetically mapped a mutation in NLRP7 as a cause for recurrent molar pregnancies with abnormal methylation patterns. The goal of this study is to identify human proteins that interact with NLRP7, especially those that are involved in DNA acetylation and methylation, which could lead to the development of molar pregnancies. A yeast two-hybrid system using a human cDNA library was used to screen for interacting proteins and protein interactions were verified by co-immunoprecipitation in HEK 239T cells. Additional experiments are being performed to determine the change in global methylation when NLRP7 and EBP1 interact. Yeast screening results indicated several proteins that interact with NLRP7, however further testing will be focused on the interaction with EBP1, a human protein involved in growth regulation. Having potentially identified a protein involved in development through acetylation/deacetylation that interacts with NLRP7, we hope to shed more light on the molecular pathways that lead to the formation of recurrent molar pregnancies.
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