NK-like and oxidative burst activities are the main early cellular innate immune responses activated after virus inoculation in reservoir fish

Abstract

Viral diseases are a major problem in fish farming and a deeper knowledge of the immunological mechanisms playing a part in the antiviral defence is still important. Moreover, fish farming practices (high densities, new areas of culture and egg/larvae/adult transport) are significantly increasing the spread of viruses and the number of susceptible or reservoir fish species. In this last point, no studies have focused on the immunological mechanisms playing a part in the antiviral responses in reservoir and non-susceptible fish species. Thus, we have evaluated the very early innate immune responses of gilthead seabream ( Sparus aurata) to the virus causing viral haemorrhagic septicaemia (VHSV) in salmonids since this virus has been found in seabream and neighbouring farmed marine fish species acting as a viral reservoir. The virus was detected in liver, head-kidney, spleen and peritoneal cavity suggesting that the virus reached these tissues but did not replicate as viral expression was almost absent by 72 h post-inoculation. Interestingly, VHSV provoked an influx of leucocytes to the peritoneal cavity and a redistribution of peritoneal exudate (PELs) and head-kidney (HKLs) leucocytes and their innate immune responses (non-specific cytotoxic (NCC or NK-like) activity, phagocytosis, reactive oxygen intermediate (ROI) production and myeloperoxidase (MPO) activity) were generally increased demonstrating that the immune system is activated and involved in the clearance of the virus. Strikingly, NK-like, ROI and MPO were the most enhanced by the presence of VHSV in both PELs and HKLs suggesting that these early innate immune events are crucial during early viral infection stages in non-susceptible or reservoir species. Differences in the immunological mechanisms between susceptible and reservoir species and with other particulate antigens are discussed.