Nitroxyl Radicals for Studying Electron Transfer

Abstract

Electron-transfer (ET) rates are measured by use of time-resolved EPR spectroscopy, involving photooxidation of nitroxyl radicals by a ruthenium bipyridyl complex. This permits acquisition of the fundamental characteristics of ET in solution. The method was used on two spin-labeled derivatives of bacteriorhodopsin, and is applicable to proteins, nucleic acids, and biological membranes. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.