Nitrous oxide production and nitrification kinetics by natural bacterial communitites of the lower Seine river (France)

Abstract

Nitrous oxide (N 2 O) is produced in the lower Seine river at 40 to 60 kg N-N 2 O d -1 , in a section greatly affected by effluents from the Acheres Wastewater treatment plant (WWTP). Because water column denitrification is weak in this sector, we hypothesized that N 2 O could be produced by the nitrifier-denitrification process. To understand the controls of N 2 O emission, nitrifying bacterial cultures were grown from Seine river water (in batches and continuous flow experiments). The population diversity of ammonia oxidizing bacteria (AOB) in these experiments was determined by denaturing gradient gel electrophoresis (DGGE) and found to be similar to those naturally present in the Seine. We determined nitrification kinetics of the 2 functional bacteria populations (AOB and nitrite oxidizing bacteria [NOB]). During nitrifying batch and continuous flow experiments, the N 2 O production kinetics were examined under contrasted conditions. We tested the effect of dissolved oxygen, ammonia and nitrite concentrations on the N 2 O production rate. To our knowledge, this is the first study determining the optimal concentrations of O 2 , NH 4 + and NO 2 - that will lead to maximum N 2 O production by nitrifier-denitrification of mixed nitrifying bacteria populations from natural freshwater. We tested a range of oxygen concentrations and observed a peak in N 2 O emission within the narrow range from 1.1 to 1.5 mg O 2 l -1 . We plotted the N 2 O production as a function of ammonium and nitrite concentrations under optimal dissolved oxygen conditions. The results followed the hyperbolic Michaelis-Menten type curves, and kinetics parameters (V max and K s ) were determined. The maximum N 2 O production rate (V max ) was estimated at 8 to 9 pg N-N 2 O mg C biomass1 h -1 . The half-saturation constants of nitrifier-denitrification were K s = 1.5 to 3 mg N-NH 4 + l -1 for ammonium, and K s = 1 to 4 mg N-NO 2 - l -1 for nitrite.