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Abstract
This study undertook to determine the nutritional factors influencing urea nitrogen production (UrP) and net protein synthesis (PrS) in protein-depleted anephric rats. Young growing rats were protein-depleted from 3-14 days by providing them a protein-free diet. They were then binephrectomized and an infusion begun. UrP was measured as gain in body urea pool derived from BUN and body water over the succeeding 24 hrs. PrS was either positive or negative, depending upon whether nitrogen intake exceeded or was less than UrP. All animals received 7 ml fluid/100 g BW/24 hrs IV in .07M NaCl to avoid hyponatremia, and insulin when appropriate to avoid hyperglycemia. Solutions were compared and expressed per 100 g BW/day:By analysis of variance the differences between each group were significant at 5% for UrP; for PrS the differences were not significant except between group 3 and 2, These data suggest that UrP is curtailed by glucose administration and more by adding EAA. Adding non-EAA increased UrP but did not affect PrS compared with glucose alone.
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