Abstract
Uptake hydrogenase and nitrogenase activities of Rhizobium japonicum strains PJ 17.1 and GA in association with Glycine max cvs. Fengshou and Giessen were investigated throughout the life cycles of the hosts.The growth of the two cultivars was greater with PJ 17.1 than with GA. The nitrogenase specific activities were maximum at 37 days after sowing with GA and 48 days after sowing with PJ 17.1 and decreased thereafter, almost similarly on both the cultivars. However, the uptake hydrogenase actities of PJ 17.1 were found to be maximum at 30 days.The relative efficiencies of PJ 17.1 were 1.0 at all stages while those of GA varied similarly on both the cultivars. As plants inoculated with PJ 17.1 were aging, a similar decline was observed in the activities of nitrogenase and hydrogenase. Although relative efficiency of GA varied throughout the life cycle of soybean, no correlation was seen between it and the nitrogenase specific activity.
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