Abstract

An assessment of nitrogenase activity associated with the basal and terminal portions of rice roots from seven rice cultivars was made. Results indicate a definite partitioning of the nitrogenase activity within the root and a plant growth dependent shift in the activity associated with basal and terminal portion of the roots. Nitrogenase activity associated with the root base steadily declined with plant growth, while root tip associated nitrogenase activity increased sharply. Further, the basal portion had higher nitrogenase activity in the prereproductive phase while the root tip had higher activity during reproductive and maturity stages of the plant. Microbiological analysis of plant roots indicated the association of higher populations of Azospirillum sp. and Azotobacter sp. with the root base, while the root tips had higher populations of facultative anaerobic nitrogen-fixing bacteria.

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