Abstract
Nitrogen fertilization can affect the susceptibility of Brassica napus to the telluric pathogen Plasmodiophora brassicae. Our previous works highlighted that the influence of nitrogen can strongly vary regarding plant cultivar/pathogen strain combinations, but the underlying mechanisms are unknown. The present work aims to explore how nitrogen supply can affect the molecular physiology of P. brassicae through its life epidemiological cycle. A time-course transcriptome experiment was conducted to study the interaction, under two conditions of nitrogen supply, between isolate eH and two B. napus genotypes (Yudal and HD-018), harboring (or not harboring) low nitrogen-conditional resistance toward this isolate (respectively). P. brassicae transcriptional patterns were modulated by nitrogen supply, these modulations being dependent on both host-plant genotype and kinetic time. Functional analysis allowed the identification of P. brassicae genes expressed during the secondary phase of infection, which may play a role in the reduction of Yudal disease symptoms in low-nitrogen conditions. Candidate genes included pathogenicity-related genes (“NUDIX,” “carboxypeptidase,” and “NEP-proteins”) and genes associated to obligate biotrophic functions of P. brassicae. This work illustrates the importance of considering pathogen’s physiological responses to get a better understanding of the influence of abiotic factors on clubroot resistance/susceptibility.
Highlights
Plants are exposed to a wide range of phytopathogens, such as fungi, bacteria, viruses, nematodes, or protists, which cause infectious diseases
It has been considered that the effect of N on plant diseases was dependent on the lifestyle of the pathogens (Solomon et al, 2003): the development of biotrophic pathogens would be enhanced by N, while the opposite effect would be observed for necrotrophic pathogens
Modulation of Plant Clubroot Symptoms According to the Nitrogen Supply and the Host-Plant Genotype
Summary
Plants are exposed to a wide range of phytopathogens, such as fungi, bacteria, viruses, nematodes, or protists, which cause infectious diseases. The effect of N on plant disease, described in many examples, can be opposite depending on the pathogen and on the plant host. In a review that summarized data from 114 studies, an increase, decrease, or no effect of N on plant disease was reported in 62, 42, and 10 cases, respectively (Sun et al, 2020). The different forms of N supply (ammonium NH4+ or nitrate NO3−) can have various effects on plant disease severity because of the use of different assimilation and metabolism pathways (Bolton and Thomma, 2008; Mur et al, 2016)
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