Abstract
To solve the thermodynamic limitations on the hydrogen (H2) yield by dark fermentation (DF), the conductive carbons are usually used to mediate the H2-DF.In this work, the nitrogen (N)-phosphorus (N) doped starch carbon (NPSC) was prepared and characterized to investigate its influence on H2-DF. NPSC effectively raise H2 yield compared with starch-derived carbon (SC). The optimal dosage of SC (400 mg/L) and NPSC (600 mg/L) caused the highest H2 yield of 219.5 and 261.2 mL/g glucose, respectively, being higher than the control yield (161.4 mL/g glucose). Factually, compared with the control group without any carbon, NPSC optimized the microbial community structure and increased the abundance of C. butyricum from 19.09% to 30.87%. This fact increased the shift of metabolic pathway to butyric acid evolution, thereby promoting the substrate conversion level to H2.
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