Abstract
ABSTRACTA simple procedure is described for the separation of nitrate, amino acid and soluble protein pools in small volumes of plant extract. Recent developments in mass spectrometry and automated, on‐line sample preparation facilitate the precise analysis of the 15N content of samples containing low concentrations of N (≤5 to ≥50μg) with enrichments close to the natural abundance level. When combined, these separation and analysis techniques make it possible to investigate and quantify the relationships between nitrogen uptake, storage, assimilation, and partitioning between discrete pools of N within individual plants, following 15N labelling.
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