Abstract
Tobacco plants (Nicotiana tabacum cv. Sevilla) were grown under controlled conditions and submitted to regular fertilization with macro- and micronutrients. The aim of the experiment was to determine the response of NO3- assimilation to four different boron (B) doses (B1: ; B2: 5 μM; B3: ; and B4: ). The content of foliar B and NO3-, the activity of the enzymes related to the process of NO3- reduction (NR, NiR, GS, GOGAT, and PEPC), and the end products of this process (amino acids and proteins) were analyzed. Our results indicate a positive effect of the B on its concentration in leaves and on NO3- assimilation, possibly by an activation of the enzymatic proteins involved in this process. Boron addition significantly reduced the foliar NO3- concentration. Finally, B affected plant growth, since an increase in leaf dry weight and in the content of proteins and amino acids was shown.
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