Nitrogen level induces sex-specific cadmium phloem remobilization and cell wall segregation in Populus cathayana

Abstract

Nitrogen (N) fertilization can improve the phytoremediation of contaminated soils. However, limited information is available on the effects and mechanisms of N availability on Cadmium (Cd) phytoextraction by dioecious plants. This study employed female and male Populus cathayana to examine sex-specific long-distance transport and cell wall Cd sequestration. Females had a greater ability to transport Cd from roots to shoots and accumulated more Cd in leaves, but had less Cd bound to the cell wall and S-containing ligands than males, irrespective of N availability. N availability affected the sex-specific ability to transport Cd and chelate it within cell walls and with S-containing ligands. Low N promoted phloem-mediated upward and downward Cd transport and total Cd accumulation in both sexes, and such effects on phloem-mediated downward Cd transport were greater than those on upward Cd transport in males. However, low-N concentration-induced Cd phloem transport was more significant in females than males. In females, low N reduced Cd accumulation in leaves via increased phloem-mediated Cd downward transport, and this Cd was subsequently sequestered in the bark and root cell walls. In contrast, for males, high N promoted xylem-mediated Cd transport to shoots and Cd sequestration in the bark but reduced phloem-mediated Cd downward transport and subsequent sequestration in root cell walls. Sex-specific genes related to root Cd transport and translocation from roots to shoots were also affected by N supply in roots. These results suggested that N availability reduced the sex-based difference in total Cd accumulation, translocation and Cd detoxification, and males showed stronger Cd tolerance than females at both N availabilities.