Nitrogen isotope fractionation in amino acids from harbor seals: implications for compound-specific trophic position calculations

Abstract

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 482:265-277 (2013) - DOI: https://doi.org/10.3354/meps10257 Nitrogen isotope fractionation in amino acids from harbor seals: implications for compound-specific trophic position calculations Leslie R. Germain1,*, Paul L. Koch2, James Harvey3, Matthew D. McCarthy1 1Department of Ocean Sciences, University of California, Santa Cruz, California 95065, USA 2Department of Earth and Planetary Sciences, University of California, Santa Cruz, California 95065, USA 3Department of Vertebrate Ecology, Moss Landing Marine Laboratories, Moss Landing, California 95039, USA *Email: leslieroland@gmail.com ABSTRACT: Compound-specific isotope analysis of individual amino acids (AA) is a rapidly growing tool in ecological studies to assess diet and trophic position (TP) in both modern and ancient foodwebs. We conducted the first controlled feeding study examining δ15N values in AAs in a marine mammal (harbor seal Phoca vitulina). The pattern of δ15N variation among AAs in seals was similar to that observed in other heterotrophs, although exceptions were found with proline and threonine. However, many δ15N changes with trophic transfer were very different than those reported for zooplankton and other lower TP marine consumers. In particular the measured trophic enrichment factor (TEF) now broadly used for TP estimation (TEFGlu-Phe) was much lower in harbor seals (~4.3‰) than the current commonly applied value (~7.5‰). Recently published data on wild marine birds (penguins) and elasmobranchs (stingrays) suggests that similar, low TEF values may also be characteristic of these taxa. Together, these data imply that marine mammals and other higher animals have different, but also diagnostic, changes in δ15N-AA with trophic transfer vs. organisms examined in previous feeding studies (e.g. zooplankton, bony fish and mollusks), possibly due to dietary protein content, trophic position, and/or form of nitrogen excretion (urea vs. ammonia). Therefore, we propose that for marine mammals, a multi-TEF calculation is required to account for variations of TEF between animals within a food web, and we demonstrate that this approach can predict accurate TP estimates for harbor seals. These results also have significant implication for the application of compound-specific isotope analysis of AAs on terrestrial ecology and trophic structure. KEY WORDS: Compound-specific isotope analysis · Trophic enrichment factors · Phoca vitulina · Trophic position · TEF · Urea · Ammonia · Protein content Full text in pdf format Supplementary material PreviousNextCite this article as: Germain LR, Koch PL, Harvey J, McCarthy MD (2013) Nitrogen isotope fractionation in amino acids from harbor seals: implications for compound-specific trophic position calculations. Mar Ecol Prog Ser 482:265-277. https://doi.org/10.3354/meps10257 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 482. Online publication date: May 22, 2013 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2013 Inter-Research.