Nitrogen form regulates cadmium uptake and accumulation in Tanzania guinea grass used for phytoextraction

Abstract

Plants benefit from the simultaneous uptake of nitrate (NO3−) and ammonium (NH4+), which can influence the bioaccumulation of heavy metals. Nevertheless, there are no studies on the effect of nitrogen forms on grasses used for cadmium (Cd) phytoextraction. The objective was to evaluate the response of Panicum maximum cv. Tanzania to NO3−/NH4+ ratios and Cd levels. A 3 × 3 factorial experiment was conducted under greenhouse conditions, in a randomized complete block design replicated three times, with three NO3−/NH4+ ratios (100/0, 70/30 and 50/50) and three Cd levels (0.0, 0.5 and 1.0 mM). Although it increased plant total nitrogen concentration, Cd exposure reduced shoot and root growth. Moreover, it altered nitrogen metabolism and induced the accumulation of NO3− and NH4+ mainly in shoots. The supply of 50/50 disturbed glutamine synthetase activity and changed root morphology under Cd toxicity. However, while the exclusive use of NO3− mitigated toxicity symptoms, by favoring Cd accumulation in roots and maintaining normal nitrogen metabolism, plants grown with 50/50 showed increased uptake, transport and accumulation of this metal. Thus, Cd uptake and accumulation are strongly related to the form of nitrogen available, and the supply of 50/50 increases the phytoextraction of this metal.