Abstract

The first succesful isolation of Frankia from the nodules of Polish Alnus glutinosa have been described. The fine structures of endophyte (thick-walled spherical vesicles of 6 μm diameter, numerous sporangia, various in shape, 60–80 μm length, aseptate and highly branched filaments 1–3 μm) have been studied by light and scanning microscopy. The pure culture in BAP medium without combined nitrogen showed nitrogenase activity (94.51 nmoles of ethylene was formed from acetylene per 1.0 ml of packed cells per hr). The isolate was capable of forming N 2-fixing symbiotic root nodules on Alnus glutinosa seedlings.

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