Abstract
INVESTIGATIONS of the symbioses in root-nodules of legumes and non-legumes have established that nitrogen fixation occurs in the nodular tissue1. Although some reports have appeared describing the leaf-nodule symbiosis in Psychotria2–4 no isolate of the endophyte is at present available, and it has not been unequivocally established that nitrogen fixation is involved. We now report that the isolated endophyte fixes atmospheric nitrogen in pure culture and, apparently, in the plant as well. It is known that plants either naturally or experimentally freed of the endophyte are dwarfs2,3. Nitrogen fixation does not fully account for these observed effects.
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