Nitrogen enrichment mediates the effects of high temperature on the growth, photosynthesis, and biochemical constituents of Gracilaria blodgettii and Gracilaria lemaneiformis.

Abstract

Gracilaria blodgettii and Gracilaria lemaneiformis are often adopted as tools to purify aquaculture tail water. However, there has been such phenomenon that high temperature in summer restricts the process of aquaculture. To explore the adaptive capacity of G. blodgettii and G. lemaneiformis, we experimented them and cultured for 12days under three temperatures (20, 25, and 30°C) and three levels of multiple nitrogen sources (0.12, 0.6 and 4.4mgL-1). Their growth, photosynthetic characteristics, and biochemical compositions including the contents of pigments and soluble protein were determined to investigate the single and interactive effects of temperatures and nitrogen levels on these two species. The results showed that in terms of G. blodgettii, the higher growth rate and more pigment (chlorophyll a and carotenoids) contents were observed at 25 and 30°C in comparison to 20°C, and the pigments showed maximum contents at 25°C. More nitrogen improved the growth rate, net photosynthetic rate (Pn) at 25 and 30°C, Fv/Fm at 20°C, maximal photosynthetic electron transfer rate (ETRm), as well as soluble protein content at 20 and 25°C. Additionally, the growth rate, Pn, and ETRm of G. lemaneiformis all showed a decline as increasing temperature; analogously high nitrogen concentration increased the growth rate at 25 and 30°C, Fv/Fm at each temperature, ETRm, and pigments contents at 20°C, as well as soluble protein content at 20 and 25°C. Conclusions indicated that high temperature restricted the growth rate, inhibited photosynthetic characteristics, and decreased the soluble protein content of G. lemaneiformis. The reduced photosynthetic performance, pigments, and soluble protein contents of G. blodgettii were noted under similar conditions. However, nitrogen enrichment induced the greater resistant level to high temperature, and G. blodgettii showed better response. These findings suggested that these two Gracilaria species possessed a certain adaptability to tail water from aquaculture at high temperature and G. blodgettii can resist more to. Therefore, it seems to be an alternative and workable scheme to adopt some suitable macroalgae to optimize the solution to present purification of aquaculture wastewater or eutrophic waters under high temperature.