Nitrogen digestion and metabolism in sheep consuming diets containing contrasting forms and levels of N.

Abstract

Nitrogen kinetics were studied in six sheep (45-55 kg live weight) consuming either a high-N grass silage or a low-N dried grass made from swards of perennial ryegrass (Lolium perenne). The diets were fed hourly at a level of 600 g dry matter/d and supplied 19.5 and 11.0 g N/d respectively. The amounts of organic matter (OM) consumed and flowing at the duodenum and ileum and excreted in the faeces were similar (P greater than 0.05) with both diets. Each diet supplied 23 g digestible OM/d per kg live weight 0.75, which was sufficient to maintain body-weight. There were no differences (P greater than 0.05) between diets in rumen fluid volume, fractional outflow rate of fluid from the rumen, total concentration of volatile fatty acids or molar proportion of acetate in the rumen. The pH and molar proportion of propionate in rumen fluid were higher (P less than 0.01), and molar proportion of butyrate lower (P less than 0.001) when the silage was given. There was a net loss of N (4.0 g/d) between mouth and duodenum when the silage was consumed but a net gain (5.5 g/d) when the dried grass was consumed. As a result, total non-ammonia-N (NAN) flow at the duodenum did not differ (P greater than 0.05) between diets. Rumen microbial NAN flow at the duodenum, based on 15N as the marker, also did not differ (P greater than 0.05) between diets but the efficiency of microbial N synthesis in the rumen (g/kg OM apparently digested) was higher (P less than 0.05) with the dried grass. When the sheep were consuming silage they had a higher concentration of ammonia in rumen fluid (P less than 0.01), a higher rate of irreversible loss of ammonia from the rumen (P less than 0.05) and a higher rate of absorption of ammonia across the rumen wall (P less than 0.01). The rate of absorption was found to be more closely related to the unionized ammonia concentration in rumen fluid (r2 0.85) than to the total ammonia concentration (r2 0.36). Endogenous N entry into the forestomachs was calculated to be 5.5 g/d when the silage was given and 9.4 g/d when the dried grass was given, of which 1.7 and 3.5 g/d respectively were in the form of urea. Thus, approximately 4-6 g N/d were derived from non-urea materials.(ABSTRACT TRUNCATED AT 400 WORDS)