Abstract
Nakai & Chi Le (1970) have described a rapid method for the determination of the N content of bovine milk based on the close correlation of absorbance at 280 nm with values for total N as determined by the Kjeldahl procedure. The applicability of the method to human milk samples has been investigated. The procedure is limited by: (1) the dependence of the relation between absorbance value and N content on both the mode of collection and the postnatal age of the donor, and (2) variable interference by non-protein components of the milk. In spite of these shortcomings, the method may be of value as a screening test for certain human milk banks.
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