Abstract
Experimental treatments designed to affect the pathway of nitrogen assimilation were applied to the roots of intact maize plants growing with ammonium or nitrate as N-sources. The treatments comprised: external amino acid feeding; specific inhibitors of GS, GOGAT, and aminotransferases; and N-starvation. Their actions in vivo were confirmed by 14 N- or 15 N-N spectroscopy, or by HPLC analysis of tissue extracts. A strong correlation was noted between the levels of glutamine and asparagine in the roots. Rates of ammonium and nitrate absorption by the plants were then measured, over both short (5 min) and longer (1.5 h) periods
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