Nitrogen and water addition regulate soil fungal diversity and co-occurrence networks

Abstract

A field experiment was conducted to assess the role of nitrogen (N) and water addition in shaping soil fungal communities and co-occurrence networks in temperate grassland, northern China. We measured soil fungal and plant community compositions, and also soil properties including available N, phosphorus, potassium concentrations, soil pH, and soil moisture. Soil fungal co-occurrence networks were constructed using a random matrix theory–based network inference approach. Plant species richness was decreased by N addition but increased by water addition, whereas fungal richness was decreased by N addition. The fungal community composition was significantly changed by both N addition and water addition. Soil fungal α diversity and β diversity were explained by a combination of variations in plant species richness and plant functional composition, and also by changes in soil pH via the soil acidification pathway induced by N and water addition. The fungal co-occurrence networks were more complex and clustered under water addition than that in ambient precipitation. Our results suggested that plant functional composition, plant species richness, and soil acidification should be incorporated into ecosystem models for predicting soil fungal communities under future climate changes in terrestrial ecosystems.