Nitrogen Addition Decreased Respiration and Heterotrophic Respiration but Increased Autotrophic Respiration in a Cabbage (Brassica pekinensis Rupr) Experiment in the Northeast Plains

Abstract

Farmland soil respiration (Rs) significantly impacts the global carbon (C) cycle. Although nitrogen (N) can promote crop growth and increase yields, its relationship with Rs and its constituents, including autotrophic respiration (Ra) and heterotrophic respiration (Rh), remains unclear. Therefore, a field study was carried out in a cabbage (Brassica pekinensis Rupr) system to probe the impact of N addition on Rs, Ra, and Rh. Five levels of N addition, including 0 kg N hm−2·yr−1 (N0), 50 kg N hm−2·yr−1 (N50), 100 kg N hm−2·yr−1 (N100), 150 kg N hm−2·yr−1 (N150), and 200 kg N hm−2·yr−1 (N200), started in March 2022. The Rs (Ra and Rh) and soil samples were measured and collected twice a month. The findings revealed the following: (1) N fertilizer enhanced Ra while reducing Rs and Rh; (2) soil temperature (ST), belowground net primary productivity (BNPP), soil inorganic N (SIN), and soil total C/total N (C/N) were the significant elements influencing Ra, and microbial biomass carbon (MBC), SIN, and microbial diversity (MD) were the primary factors influencing Rh; (3) partial least squares-path models (PLS-PM) showed that ST and SIN directly impacted Rh, while ST and BNPP tangentially influenced Ra; (4) 150 kg N hm−2·yr−1 was the ideal N addition rate for the cabbage in the region. In summary, the reactions of Ra and Rh to N fertilizer in the Northeast Plains are distinct. To comprehend the underlying processes of Rs, Ra, and Rh, further long-term trials involving various amounts of N addition are required, particularly concerning worsening N deposition.