Abstract
AbstractSeveral budding scientists and others have often attempted writing secret messages on paper, either for fun or sending secret messages to friends. These science nerds used lemon juice, milk, or other solutions that could be used with a dip pen, brush, or a fountain pen to write invisible messages on a blank white paper. Words appear as one exposes the paper to heat in one form or the other, as though by magic. One study used nitrocellulose membranes for secret messaging using alkaline phosphatase as the invisible ink, followed by magical visualization of the message using nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate and chemiluminescence detection systems. This chapter details the method described by Jasmin Kurien and Bianca Kurien to use nitrocellulose membrane as the new canvas for art. Their work showed that nitrocellulose membrane could serve as a canvas for art using alkaline phosphatase as the invisible ink and then detecting the art magically with the NBT/BCIP detection system.KeywordsNitrocellulose membraneArtCanvasAlkaline phosphataseNBT/BCIP
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