Abstract
An instantaneous increase in reactor free ammonia concentration resulted in a gradual decrease in nitrite oxidizer activity that was coincidental with an increase in the nitrous oxide emissions from the reactor. Microbial community analysis using RNA slot blotting techniques indicated that Nitrospira, and not Nitrobacter, populations appeared to be most affected by this induced perturbation. It is hypothesized that competition for nitrite and possibly oxygen between ammonia oxidizers and Nitrospira, and not free ammonia stress, was the true cause of impaired nitrite oxidizer activity. Key words: ammonia inhibition, fatty acid methyl esters, free ammonia, molecular methods, nitrification, nitrite, Nitrobacter, Nitrospira, nitrous oxide, RNA slot blotting, wastewater treatment.
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