Abstract
We investigated nitric oxide (NO) synthase activity in cultured neonatal rat cardiac myocytes and fibroblasts upon treatment with interleukin 1β (IL-1β) and lipopolysaccharide (LPS). Incubation of cardiac myocytes for 24 h with IL-1β or LPS caused a significant increase in NO and cGMP production. Simultaneous incubation of IL-1β with N G-monomethyl-L-arginine or transforming growth factor β (TGF-β) completely inhibited the IL-1β-induced NO and cGMP production in cardiac myocytes. In contrast, incubation of cardiac fibroblasts for 24 h with Il-1β or LPS showed no significant effect on NO or cGMP production. Addition of IL-1gb decreased the beating rate of cardiac myocytes, but TGF-β overcame that inhibition. These observations suggest the presence of iNOS in cardiac myocytes, which is an important regulator of contractile function of the heart.
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