Nitric oxide synthase in the olfactory center of the snail, Helix pomatia. A light- and electronmicroscopic histochemical study

Abstract

Event Abstract Back to Event Nitric oxide synthase in the olfactory center of the snail, Helix pomatia. A light- and electronmicroscopic histochemical study K. Nacsa1, K. Elekes1 and Z. Serfőző1* 1 Balaton Limnological Research Institute, Hungarian Academy of Sciences, Department of Experimental Zoology, Hungary Nitric oxide (NO) contributes to the generation of oscillatory waves which are related to the process of memory formation in the procerebrum (PC), the olfactory center of terrestrial snails. The anatomy of NO synthase (NOS) containing neurons is known very little in the PC. Therefore in this study we localized NOS using both NADPH-diaphorase (NADPH-d) histochemical reaction (NADPH-d) and NOS immunohistochemistry in the PC of Helix pomatia. Labeling alternate cryostat sections obtained from the PC revealed an identical NADPH-d and NOS localization; NOS was detected both in the globuli cell bodies and axons, and the different neuropil areas. A gradual from top to base appearance of NOS was seen in the terminal neuropil, whereas it was evenly and strongly expressed in the internal neuropil. A lateral neuropil region receiving NOS positive axon terminals both from the upper and lower tentacles was also intensively labeled. At ultrastructural level NADPH-d cytochemistry revealed that NOS was bound to the nuclear envelope and endoplasmic reticulum elements in the globuli cells, whereas it was attached to the membrane of agranular synaptic vesicles and medium electron dense granular vesicles in the neuropil. These observations refer to the synthesis of NO in globuli cells, and support the involvement of NO both in the process of transmitter release and intercellular signaling in the different neuropil regions. Acknowledgements Support: OTKA, Nos. 78224 (K.E.), PD75276 (Z.S.) Keywords: Sensory and motor activity, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Sensory and motor activity Citation: Nacsa K, Elekes K and Serfőző Z (2011). Nitric oxide synthase in the olfactory center of the snail, Helix pomatia. A light- and electronmicroscopic histochemical study. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00003 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. Z. Serfőző, Balaton Limnological Research Institute, Hungarian Academy of Sciences, Department of Experimental Zoology, Tihany, Hungary, serfozo@tres.blki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers K. Nacsa K. Elekes Z. Serfőző Google K. Nacsa K. Elekes Z. Serfőző Google Scholar K. Nacsa K. Elekes Z. Serfőző PubMed K. Nacsa K. Elekes Z. Serfőző Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.